Vol. 44 (6): 1234-1242, November – December, 2018

doi: 10.1590/S1677-5538.IBJU.2017.0656


Muhammed A. P. Manzoor 1, Surya Ram Duwal 2, M. Mujeeburahiman 3, Punchappady-Devasya Rekha 1
1 Yenepoya Research Centre, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India; 2 Department of Biochemistry, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India; 3 Department of Urology, Yenepoya Medical College, Yenepoya University, Mangalore, Karnataka, India


Background: Formation of struvite stones is associated with urinary tract infection by urease-producing bacteria. Biogenic crystal growth in natural and synthetic materials is regulated by the action of inhibitors, ranging from small ions, molecules to large macromolecules.

Materials and Methods: We report the dynamics of in vitro crystallization of struvite in presence of vitamin C in synthetic urine using single diffusion gel growth technique. Sodium metasilicate gel of specific gravity 1.05 and the aqueous solution of ammo­nium dihydrogen phosphate were used as the medium for growing the struvite crystals. The crystallization process was induced by a urease positive struvite stone associated Pseudomonas aeruginosa to mimic the infection leading to stone formation. The grown crystals were characterized by ATR-FTIR and powder XRD. The surface morphology was analysed through FE-SEM for comparison between treatments.

Results: We observed decrease in number, dimension, and growth rate of struvite crys­tals with the increasing concentrations of vitamin C. Crystals displayed well-defined faces and dendritic morphology of struvite in both control and biogenic systems.

Conclusion: The results strongly suggest that, vitamin C can modulate the formation of struvite crystals in the presence of uropathogenic bacteria.


Keywords: Struvite; Pseudomonas aeruginosa; Ascorbic Acid

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