Vol. 45 (6): 1249-1259, November – December, 2019

doi: 10.1590/S1677-5538.IBJU.2019.0167


Rouhi Afkari 1, Mohammad Mehdi Feizabadi 2, Alireza Ansari-Moghadam 3, Tahereh Safari 4, Mohammad Bokaeian 1
1 Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran; 2 Tehran University of Medical Sciences (TUMS), Tehran, Iran; 3 School of Health, Health Promotion Research Center, Zahedan University of Medical Sciences, Zahedan, Iran; 4 Zahedan University of Medical Sciences, Zahedan, Iran


Objective: Urinary stones with oxalate composition can cause kidney failure. Recent findings evidenced that probiotics are effective in reducing oxalate absorption in these subjects based on their high colonic absorption levels at baseline. The purpose of this study was to evaluate the effect of the simultaneous use of oxalate-degrading bacteria, Urtica dioica and T. terrestris extract in reducing urinary oxalate.
Materials and Methods: Anti-urolithiatic activity of Urtica dioica and T. terrestris ex­tract and probiotic by using ethylene glycol induced rat model. In this study, 4 strains of Lactobacillus and 2 strains of Bifidobacterium and also 2 strains of L. paracasei (that showed high power in oxalate degrading in culture media) were used. Male Wistar rats were divided into four groups (n=6). The rats of group-I received normal diet (positive control group) and groups-II (negative control group), III, IV rats received diet con­taining ethylene glycol (3%) for 30 days. Groups III rats received Urtica dioica and T. terrestris extract. Groups IV rats received extracts + probiotic for 30 days.
Findings: The results show that the use of herbal extracts (Urtica dioica and T. ter­restris) reduced the level of urinary oxalate and other parameters of urine and serum. Also, the accumulation of calcium oxalate crystals in the kidney tissue was signifi­cantly reduced.
Conclusion: Considering that the formation of calcium oxalate crystals can cause in­flammation and tissue damage in the kidney, the use of herbal extracts with oxalate degrading bacteria can be a new therapeutic approach to preventing the formation of kidney stones.

Keywords: Probiotics; Jeevaneeya Rasayana [Supplementary Concept]; cationic protein, urate-calcium oxalate stone, human [Supplementary Concept]

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